Anti-human IgE mAb (107), unconjugated

Anti‑human IgE mAb (107), unconjugated

3810-3-250

Publications: 22
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Validated for our assays.

Content

Monoclonal antibody 107, unconjugated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

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Product specifications

Intended use

This monoclonal antibody enables specific detection of human IgE in immunoassays such as ELISA and ELISpot.

Serum/Plasma samples

Recommendation

107 is recommended as capture mAb in ELISA in combination with detection mAb 182 (product code 3810-6) and with detection mAbs 107/182/101 (product code 3810-8) for ELISpot.

Product details

ProductAnti-human IgE mAb (107), unconjugated
ApplicationELISA, ELISpot
AnalyteIgE
Antibody107
ConjugateUnconjugated
ClonalityMonoclonal
ImmunogenNative human IgE
HostMouse
IsotypeIgG1
ReactivityHuman
Specificity

Human IgE

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Concentration1 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody 107, unconjugated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C.

Shelf lifeAt least 18 months from date of receipt.

Selecting the appropriate mAbs is key to detecting both total and antigen-specific human IgA. This blog post explores Mabtech’s mAbs, tailored for ELISpot to detect human IgA antibody-secreting cells.

 

Application guide: ELISA

 

Antigen-specific IgE

Coating:
Antigen
Detection mAbs:
107/182/101, biotin

IgE Ag

Total IgE

Coating:
107, unconjugated
Detection mAbs:
182, biotin

IgE total

 

Application guide: ELISpot

 

Total IgE

Coating mAb:
107, unconjugated
Detection mAbs:
107/182/101, biotin

ELISpot IgE

 

 

Get inspired!

Make sure to check out how researchers have used Mabtech's human IgE ELISA, ELISpot, and FluoroSpot kits in allergy research. You can find it all in our Publication database.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IgE

Analyte description

The main physiological function of immunoglobulin E (IgE) is to protect against parasitic infestation. However, IgE is also associated with allergy, and allergen-specific IgE antibodies may trigger allergic reactions in the presence of the allergen. IgE is normally found in low amounts in serum/plasma but is significantly increased in allergic individuals, and the detection of allergen-specific IgE serves as the basis for many in vitro assays for allergy diagnosis.

Alternative namesImmunoglobulin E, IgE
Cell typeB cell
Gene ID3497
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